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KMID : 0364820120480040262
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.262 ~ p.269
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Seasonal Differences of Bacterial Communities Associated with the Marine Sponge, Hymeniacidon sinapium
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Jung Jong-Bin
Park Jin-Sook
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Abstract
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Seasonal differences of the cultivable bacterial communities associated with the marine sponge, Hymeniacidon
sinapium, between spring and summer were analyzed through the Amplified Ribosomal DNA Restriction Analysis
(ARDRA). For the cultivation of the bacterial isolates, modified Zobell and MA media were used. The 16S rDNA of
individual strains were amplified and fragmented by using two restriction enzymes, HaeIII and MspI. As a result, 23
ARDRA types from the spring sponge and 28 types from the summer sponge were obtained. The partial sequencing
result of 1 to 3 selected strains from each types showed over 94% similarities with the known species from the
public database. The bacterial communities from the sponge, captured on spring, contained 4 phyla: Actinobacteria,
Alphaproteobacteria, Gammaproteobacteria, and Firmicutes. There were 5 phyla observed from the bacterial
communities associated with the sponge, captured on summer: Alphaproteobacteria, Betaproteobacteria,
Gammaproteobacteria, Firmicutes, and Bacteroidetes. Gammaproteobacteria was predominant group in both spring
and summer, accounted for 33.8% of total in spring and 67.4% in summer, showed increase pattern on summer.
Because Firmicutes and Actinobacteria participated in 30.2% and 8.3% of the spring sponge while they represented
only 6.9% and 0% of the summer sponge, both bacterial groups showed decrease drift on summer.
Betaproteobacteria (4.7%) and Bacteroidetes (4.7%) were only observed on the sponge captured on summer. On the
sponge, Hymeniacidon sinapium, more diverse bacterial communities were shown on summer than on spring, and
even from the same sponge, there were seasonal differences
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KEYWORD
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16S rDNA, Gammaproteobacteria, Hymeniacidon sinapium, ARDRA, sponge
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